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quantikine elisa kits  (R&D Systems)


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    R&D Systems quantikine elisa kits
    Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdf+15/Human+GDF-15+Quantikine+ELISA+Kit/pmc13624431-104-6-9
    Average 96 stars, based on 287 article reviews
    quantikine elisa kits - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Risk Stratification of Acute Chest Pain in Patients With High‐Sensitivity Troponin T Below the 99th Percentile: A Long‐Term Cohort Study Assessing the Incremental Value of Necrosis and Non‐necrosis Biomarkers to Clinical Risk Scores
    Article Snippet: .. HFABP (heart- type fatty acid binding protein; automated immunoturbidimetric immunoassay from Randox Laboratories) and GDF- 15 (growth differentiation factor 15; quantitative sandwich enzyme immunoassay technique using the Quantikine ELISA Human GDF- 15 kit from R&D Systems) were measured using local research and clinical laboratories. .. Galectin- 3, hs- CRP (high- sensitivity C- reactive protein), NT- proBNP (N- terminal prohormone of brain natriuretic peptide) and hs- cTnI (high- sensitivity cardiac troponin I) were determined using ARCHITECT immunoassays (Abbott Diagnostics) conducted by Abbott Laboratories, Illinois, USA.

    Article Title: Risk Stratification of Acute Chest Pain in Patients With High‐Sensitivity Troponin T Below the 99th Percentile: A Long‐Term Cohort Study Assessing the Incremental Value of Necrosis and Non‐necrosis Biomarkers to Clinical Risk Scores
    Article Snippet: .. HFABP (heart‐type fatty acid binding protein; automated immunoturbidimetric immunoassay from Randox Laboratories) and GDF‐15 (growth differentiation factor 15; quantitative sandwich enzyme immunoassay technique using the Quantikine ELISA Human GDF‐15 kit from R&D Systems) were measured using local research and clinical laboratories. .. Galectin‐3, hs‐CRP (high‐sensitivity C‐reactive protein), NT‐proBNP (N‐terminal prohormone of brain natriuretic peptide) and hs‐cTnI (high‐sensitivity cardiac troponin I) were determined using ARCHITECT immunoassays (Abbott Diagnostics) conducted by Abbott Laboratories, Illinois, USA.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Risk Stratification of Acute Chest Pain in Patients With High‐Sensitivity Troponin T Below the 99th Percentile: A Long‐Term Cohort Study Assessing the Incremental Value of Necrosis and Non‐necrosis Biomarkers to Clinical Risk Scores
    Article Snippet: .. HFABP (heart- type fatty acid binding protein; automated immunoturbidimetric immunoassay from Randox Laboratories) and GDF- 15 (growth differentiation factor 15; quantitative sandwich enzyme immunoassay technique using the Quantikine ELISA Human GDF- 15 kit from R&D Systems) were measured using local research and clinical laboratories. .. Galectin- 3, hs- CRP (high- sensitivity C- reactive protein), NT- proBNP (N- terminal prohormone of brain natriuretic peptide) and hs- cTnI (high- sensitivity cardiac troponin I) were determined using ARCHITECT immunoassays (Abbott Diagnostics) conducted by Abbott Laboratories, Illinois, USA.

    Article Title: Circulating Biomarkers and Recurrence of Persistent Atrial Fibrillation After Electrical Cardioversion
    Article Snippet: .. Serum was used for the determinations of MMP-9, TIMP-1, GAL-3, GDF-15, and IL-6, all measured using ELISA kits from Bio-Techne, R&D Systems Europe (Abingdon, Oxon, UK; Human Quantikine MMP-9, Human Quantikine TIMP-1, Human Quantikine Galectin-3, Human Quantikine GDF-15, High sensitivity Quantikine Human IL-6) and ST2, measured by Presage ST2 Assay (Critical Diagnostics, San Diego, US). .. EDTA plasma was used to determine Human Quantikine TGF-β1 with a pre-activation step (Bio-Techne) and Human Intact FGF-23 (QUIDEL, San Diego, California, USA).

    Article Title: Growth/Differentiation Factor 15 Promotes a Pro‐Regenerative Response in Chondrocytes Upon Cartilage Injury
    Article Snippet: .. To quantify GDF‐15 in synovial fluid samples and cell culture supernatants, the human GDF‐15 ELISA Kit by R&D Systems was used, according to the manufacturer`s protocol. ..

    Article Title: Risk Stratification of Acute Chest Pain in Patients With High‐Sensitivity Troponin T Below the 99th Percentile: A Long‐Term Cohort Study Assessing the Incremental Value of Necrosis and Non‐necrosis Biomarkers to Clinical Risk Scores
    Article Snippet: .. HFABP (heart‐type fatty acid binding protein; automated immunoturbidimetric immunoassay from Randox Laboratories) and GDF‐15 (growth differentiation factor 15; quantitative sandwich enzyme immunoassay technique using the Quantikine ELISA Human GDF‐15 kit from R&D Systems) were measured using local research and clinical laboratories. .. Galectin‐3, hs‐CRP (high‐sensitivity C‐reactive protein), NT‐proBNP (N‐terminal prohormone of brain natriuretic peptide) and hs‐cTnI (high‐sensitivity cardiac troponin I) were determined using ARCHITECT immunoassays (Abbott Diagnostics) conducted by Abbott Laboratories, Illinois, USA.

    Article Title: WNT3A Upregulates the RIP1/HIF-1α/GDF-15 Pathway to Induce EMT and Thereby Increase Colorectal Cancer Cell Migration and Invasion.
    Article Snippet: Stained cells were counted under a microscope, and statistical analysis was performed using the GraphPad Prism Software (GraphPad Software, Inc.). .. To confirm secretion of GDF-15 in the culture model, conditioned media from DLD-1 cells cultured in the presence of WNT3A or the control were collected and analyzed using a Human GDF-15 Quantikine ELISA Kit (R&D Systems). .. Sera obtained from colon cancer patients were analyzed using a Human Resistin Quantikine ELISA kit (R&D Systems) and a Human GDF-15 Quantikine ELISA Kit (R&D Systems).

    Article Title: Plasma biomarkers for monitoring muscle function and physical performance decline in older adults: A 2-year longitudinal study
    Article Snippet: .. An ELISA kit was used to measure the following proteins according to the manufacturer’s instructions: Human IL-6 (R&D systems, HS600C), P3NP (Aviva System Biology, OKEH00548), leptin (Millipore, EZHL-80SK), TNF-alpha (R&D systems, HSTA00E), myostatin (R&D systems, DGDF80), GDF-15 (R&D systems, DGD150), activin A (R&D systems, DAC00B), BDNF (R&D systems, DBNT00), irisin (Adipogen, AG-45A-0046YEK-KI01), adiponectin (Millipore, EZHADP-61 K), DHEAS (Siemens, L2KDS2), IGF-1 (Roche, 7475918), and cortisol (Abbott, 08P3320). ..

    Multiple Displacement Amplification:

    Article Title: New biomarkers of inflammation associated with haemodialysis
    Article Snippet: .. Moreover, commercial enzyme-linked immunosorbent assay kits were used to quantify the levels of GDF-15 (DGD150; R&D Systems, Minneapolis, MN, USA), IS (LGM-18002; Leadgene Biomedical, Taiwan), total antioxidant capacity (TAC; STA-360; Cell Biolabs, San Diego, CA, USA) and malondialdehyde (MDA; KB03016; Bioquochem, Asturias, Spain) following the protocol provided by the manufacturer. .. Total RNA, including the miRNA fraction, was extracted from serum using a commercial, column-based system (miRNeasy Serum/Plasma Advanced Kit; 217204; Qiagen, Hilden, Germany) according to the manufacturer's protocol with minor modifications.

    Cell Culture:

    Article Title: Growth/Differentiation Factor 15 Promotes a Pro‐Regenerative Response in Chondrocytes Upon Cartilage Injury
    Article Snippet: .. To quantify GDF‐15 in synovial fluid samples and cell culture supernatants, the human GDF‐15 ELISA Kit by R&D Systems was used, according to the manufacturer`s protocol. ..

    Article Title: WNT3A Upregulates the RIP1/HIF-1α/GDF-15 Pathway to Induce EMT and Thereby Increase Colorectal Cancer Cell Migration and Invasion.
    Article Snippet: Stained cells were counted under a microscope, and statistical analysis was performed using the GraphPad Prism Software (GraphPad Software, Inc.). .. To confirm secretion of GDF-15 in the culture model, conditioned media from DLD-1 cells cultured in the presence of WNT3A or the control were collected and analyzed using a Human GDF-15 Quantikine ELISA Kit (R&D Systems). .. Sera obtained from colon cancer patients were analyzed using a Human Resistin Quantikine ELISA kit (R&D Systems) and a Human GDF-15 Quantikine ELISA Kit (R&D Systems).

    Control:

    Article Title: WNT3A Upregulates the RIP1/HIF-1α/GDF-15 Pathway to Induce EMT and Thereby Increase Colorectal Cancer Cell Migration and Invasion.
    Article Snippet: Stained cells were counted under a microscope, and statistical analysis was performed using the GraphPad Prism Software (GraphPad Software, Inc.). .. To confirm secretion of GDF-15 in the culture model, conditioned media from DLD-1 cells cultured in the presence of WNT3A or the control were collected and analyzed using a Human GDF-15 Quantikine ELISA Kit (R&D Systems). .. Sera obtained from colon cancer patients were analyzed using a Human Resistin Quantikine ELISA kit (R&D Systems) and a Human GDF-15 Quantikine ELISA Kit (R&D Systems).

    other:

    Article Title: Reduced melanocortin tone mediates increased feeding during pregnancy in mice
    Article Snippet: The following pharmacological drugs and doses/routes of administration were used in this study: peptide YY (3-36) (10ug/kg, i.p.; Tocris: 1618), liraglutide (100ug/kg, s.c., Tocris: 6517), GDF-15 (10ug/kg, i.p., R&D Systems: 8944-GD), Clozapine-N-oxide (CNO, 1mg/kg, i.p.; Enzo Life Sciences: BML-NS105), Setmelanotide (100ug/kg or 300ug/kg, i.p.; 0.2ug, i.c.v.



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    Image Search Results


    (A) Proteome Profiler Human XL cytokine array (105 cytokines) of conditioned medium from proliferating, doxorubicin-induced (Dox), and Gem/Cis-induced senescent-like RBE cells. (B) Gdf15 mRNA in proliferating versus senescent-like murine CCA (SB1) cells. (C) Gdf15 mRNA in control vs AP-treated SB-p16-ATTAC tumors. (D) Schematic of in vitro studies. (E-F) Arg1 and iNOS mRNA in bone-marrow-derived macrophage (BMDM) incubated for 72 h with conditioned medium from vehicle- or alisertib-treated SB1 cells expressing shCtrl or shGdf15 . (G) Bulk RNA-seq heatmap (row Z-scores) of naïve bone marrow-derived macrophages (BMDM) cultured 72 h with conditioned media (CM) from SB-p16-shCtrl or SB- p16-Gdf15 OE cells. (H) ARG1 mRNA in human monocyte-derived macrophages ± recombinant human GDF-15 (Qkine Qk017; TGF-β1-free), qRT-PCR relative to vehicle; mean ± SEM, n = 6. (I) Division index (CFSE dilution, normalized to T-cells-only) of anti-CD3/CD28-activated CD8⁺ T cells co-cultured with BMDMs incubated for 72 h with conditioned medium from vehicle- or alisertib-treated SB1 cells expressing shCtrl or shGdf15 . Data are mean ± SEM; *, P<0.05; **, P<0.01; ****, P<0.0001; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Proteome Profiler Human XL cytokine array (105 cytokines) of conditioned medium from proliferating, doxorubicin-induced (Dox), and Gem/Cis-induced senescent-like RBE cells. (B) Gdf15 mRNA in proliferating versus senescent-like murine CCA (SB1) cells. (C) Gdf15 mRNA in control vs AP-treated SB-p16-ATTAC tumors. (D) Schematic of in vitro studies. (E-F) Arg1 and iNOS mRNA in bone-marrow-derived macrophage (BMDM) incubated for 72 h with conditioned medium from vehicle- or alisertib-treated SB1 cells expressing shCtrl or shGdf15 . (G) Bulk RNA-seq heatmap (row Z-scores) of naïve bone marrow-derived macrophages (BMDM) cultured 72 h with conditioned media (CM) from SB-p16-shCtrl or SB- p16-Gdf15 OE cells. (H) ARG1 mRNA in human monocyte-derived macrophages ± recombinant human GDF-15 (Qkine Qk017; TGF-β1-free), qRT-PCR relative to vehicle; mean ± SEM, n = 6. (I) Division index (CFSE dilution, normalized to T-cells-only) of anti-CD3/CD28-activated CD8⁺ T cells co-cultured with BMDMs incubated for 72 h with conditioned medium from vehicle- or alisertib-treated SB1 cells expressing shCtrl or shGdf15 . Data are mean ± SEM; *, P<0.05; **, P<0.01; ****, P<0.0001; ns, not significant.

    Article Snippet: Human recombinant GDF-15 was from Qkine (Qk017) and murine recombinant GDF-15 from R&D Systems (8944-GD/CF).

    Techniques: Control, In Vitro, Derivative Assay, Incubation, Expressing, RNA Sequencing, Cell Culture, Recombinant, Quantitative RT-PCR

    (A) Macrophages express TGFBR2, the candidate non-canonical GDF-15 receptor, in murine and human CCA single-cell RNA-seq. (B) α-TGFBR2 western blot (WB) of streptavidin pull-down (top) and input (middle) from naïve BMDMs treated with biotin-supplemented conditioned media (CM) from CCA cells expressing GDF-15-BioID2-HA or SEC61B-BioID2-HA (ER control); α-HA blot of CM (bottom) confirms bait secretion. (C) GDF-15 induces macrophage phospho-STAT6 but not phospho-SMAD2/3, reflecting non-canonical TGFBR2 output. (D) WB of BMDMs stimulated with control or GDF-15-overexpressing (GDF-15 OE ) CM for 0 or 1 h: p-STAT6, STAT6, p-SMAD3, SMAD3. (E) Arg1 mRNA in BMDMs transfected with siCtrl or siTgfbr2 and stimulated with control or rGDF-15. n = 6. Data are mean ± SEM; *, P<0.05; **, P<0.01; ****, P<0.0001; ns, not significant.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Macrophages express TGFBR2, the candidate non-canonical GDF-15 receptor, in murine and human CCA single-cell RNA-seq. (B) α-TGFBR2 western blot (WB) of streptavidin pull-down (top) and input (middle) from naïve BMDMs treated with biotin-supplemented conditioned media (CM) from CCA cells expressing GDF-15-BioID2-HA or SEC61B-BioID2-HA (ER control); α-HA blot of CM (bottom) confirms bait secretion. (C) GDF-15 induces macrophage phospho-STAT6 but not phospho-SMAD2/3, reflecting non-canonical TGFBR2 output. (D) WB of BMDMs stimulated with control or GDF-15-overexpressing (GDF-15 OE ) CM for 0 or 1 h: p-STAT6, STAT6, p-SMAD3, SMAD3. (E) Arg1 mRNA in BMDMs transfected with siCtrl or siTgfbr2 and stimulated with control or rGDF-15. n = 6. Data are mean ± SEM; *, P<0.05; **, P<0.01; ****, P<0.0001; ns, not significant.

    Article Snippet: Human recombinant GDF-15 was from Qkine (Qk017) and murine recombinant GDF-15 from R&D Systems (8944-GD/CF).

    Techniques: Single Cell, RNA Sequencing, Western Blot, Expressing, Control, Transfection

    (A) Strategy for p16-promoter-restricted Gdf15 silencing (shRNA) and Gdf15 overexpression. (B) qPCR validation of Gdf15 knockdown and overexpression in senescent-like SB1 cells. (C-D) Subcutaneous model and tumor-burden quantification. (E) GeoMx spatial profiling of GDF-15 expression and macrophage infiltration in human CCA. Region-of-interest (ROI)-level analysis plotting GDF-15 expression against macrophage score; each point is one ROI, with the linear fit and 95% confidence band shown. Spearman ρ = 0.63, P = 0.0067. Data are mean ± SEM; **, P<0.01; ****, P<0.0001.

    Journal: bioRxiv

    Article Title: Therapy-induced senescent-like cancer cells drive macrophage-mediated immunosuppression in cholangiocarcinoma

    doi: 10.64898/2026.06.24.734341

    Figure Lengend Snippet: (A) Strategy for p16-promoter-restricted Gdf15 silencing (shRNA) and Gdf15 overexpression. (B) qPCR validation of Gdf15 knockdown and overexpression in senescent-like SB1 cells. (C-D) Subcutaneous model and tumor-burden quantification. (E) GeoMx spatial profiling of GDF-15 expression and macrophage infiltration in human CCA. Region-of-interest (ROI)-level analysis plotting GDF-15 expression against macrophage score; each point is one ROI, with the linear fit and 95% confidence band shown. Spearman ρ = 0.63, P = 0.0067. Data are mean ± SEM; **, P<0.01; ****, P<0.0001.

    Article Snippet: Human recombinant GDF-15 was from Qkine (Qk017) and murine recombinant GDF-15 from R&D Systems (8944-GD/CF).

    Techniques: shRNA, Over Expression, Biomarker Discovery, Knockdown, Expressing

    Factors associated with GDF-15. Univariate analysis of the correlations of GDF-15 with the (a) estimated glomerular filtration rate (eGFR), (b) ln urine protein-to-creatinine ratio (UPCR), (c) ln N-terminal pro-B-type natriuretic peptide (NT-proBNP), (d) hemoglobin, (e) ln interleukin-6 (IL-6), (f) ln tumor necrosis factor-α (TNF-α), (g) albumin, and (h) lean tissue index.

    Journal: The Journal of Nutrition, Health & Aging

    Article Title: Association of growth differentiation factor-15 with muscle wasting and anemia in chronic kidney disease

    doi: 10.1016/j.jnha.2026.100835

    Figure Lengend Snippet: Factors associated with GDF-15. Univariate analysis of the correlations of GDF-15 with the (a) estimated glomerular filtration rate (eGFR), (b) ln urine protein-to-creatinine ratio (UPCR), (c) ln N-terminal pro-B-type natriuretic peptide (NT-proBNP), (d) hemoglobin, (e) ln interleukin-6 (IL-6), (f) ln tumor necrosis factor-α (TNF-α), (g) albumin, and (h) lean tissue index.

    Article Snippet: Plasma levels of GDF-15 were quantified using the Human GDF-15 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems, Minneapolis, MN, USA), with intra- and inter-assay coefficients of variation <5%.

    Techniques: Filtration

    Kaplan–Meier curves by growth differentiation factor-15 (GDF-15) median. Participants with GDF-15 concentrations above the median had a higher cumulative incidence of all-cause mortality than those with lower concentrations (log-rank P < 0.001). Numbers at risk are shown below the plot.

    Journal: The Journal of Nutrition, Health & Aging

    Article Title: Association of growth differentiation factor-15 with muscle wasting and anemia in chronic kidney disease

    doi: 10.1016/j.jnha.2026.100835

    Figure Lengend Snippet: Kaplan–Meier curves by growth differentiation factor-15 (GDF-15) median. Participants with GDF-15 concentrations above the median had a higher cumulative incidence of all-cause mortality than those with lower concentrations (log-rank P < 0.001). Numbers at risk are shown below the plot.

    Article Snippet: Plasma levels of GDF-15 were quantified using the Human GDF-15 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems, Minneapolis, MN, USA), with intra- and inter-assay coefficients of variation <5%.

    Techniques: